Composite
Part:BBa_K952006
Designed by: Saimon Sharif Group: iGEM12_Columbia-Cooper-NYC (2012-10-01)
const. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette
This part has the constitute promoter (BBa_J23102) upstream and BBa_K952002 downstream. BBa_K952002 originally did not have a promoter. This can be used immediately in a cell to cause lysis/cell death when a cell is exposed to blue-light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218
[edit]
Categories
Parameters
None |